Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis
نویسندگان
چکیده
منابع مشابه
Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis
Polo-like kinases (PLKs) are conserved eukaryotic cell cycle regulators, which play multiple roles, particularly during mitosis. The function of Trypanosoma brucei PLK was investigated in procyclic and bloodstream-form parasites. In procyclic trypanosomes, RNA interference (RNAi) of PLK, or overexpression of TY1-epitope-tagged PLK (PLKty), but not overexpression of a kinase-dead variant, result...
متن کاملCRL4WDR1 Controls Polo-like Kinase Protein Abundance to Promote Bilobe Duplication, Basal Body Segregation and Flagellum Attachment in Trypanosoma brucei
The Polo-like kinase homolog in Trypanosoma brucei, TbPLK, plays essential roles in basal body segregation, flagellum attachment and cytokinesis. The level of TbPLK protein is tightly controlled, but the underlying mechanism remains elusive. Here, we report a Cullin-RING ubiquitin ligase composed of Cullin4, the DNA damage-binding protein 1 homolog TbDDB1 and a WD40-repeat protein WDR1 that con...
متن کاملOrganelle Segregation and Cytokinesis in Trypanosoma brucei
From Division of Emerging and Transfusion Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, 20892. Department of Pharmaceutical Chemistry, University of California, San Francisco, California, 94143 Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina, 29634 Tumor Biology Program, Mayo Clinic Coll...
متن کاملPolo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means.
Polo-like kinase in Trypanosoma brucei (TbPLK) is confined to the flagellum attachment zone (FAZ) and regulates only cytokinetic initiation. However, it apparently diffuses into the cytoplasm before the trans-localization of chromosomal passenger complex (CPC) from the midzone of central spindle to FAZ, which is known to be required for initiating cytokinesis. Synchronized T. brucei procyclic c...
متن کاملPolo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei
A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single polo-like kinase (PLK) homologue in T. brucei (TbPLK). Depletion of TbPLK leads to malformed bilob...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 2007
ISSN: 0950-382X,1365-2958
DOI: 10.1111/j.1365-2958.2007.05866.x